› 8b71

Electron Microscopy
3.8Å resolution

Upright KimA dimer with bound c-di-AMP from B. subtilis

Released:
DOI: 10.2210/pdb8b71/pdb
PDB entry 8b71 coloured by chain, front view.
PDB entry 8b71 coloured by chain, side view.
PDB entry 8b71 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Cyclic di-AMP traps proton-coupled K+ transporters of the KUP family in an inward-occluded conformation.
Fuss MF, Wieferig JP, Corey RA, Hellmich Y, Tascón I, Sousa JS, Stansfeld PJ, Vonck J, Hänelt I
OpenAccess logo Nat Commun 14 3683 (2023)
PMID: 37344476
Related structures: EMD-15895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium transporter KimA Chains: A, B
Molecule details ›
Chains: A, B
Length: 607 amino acids
Theoretical weight: 66.84 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P96589 (Residues: 1-607; Coverage: 100%)
Gene names: BSU04320, kimA, ydaO
Sequence domains: Amino acid permease

Ligands and Environments

1 bound ligand:
Ligand 2BA 2 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-15895
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.
Elston et al. (2023)
1 more