› 8b7a

X-ray diffraction
2.25Å resolution

Tubulin - maytansinoid - 4 complex

Released:
DOI: 10.2210/pdb8b7a/pdb
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PDB entry 8b7a coloured by chain, front view.
PDB entry 8b7a coloured by chain, side view.
PDB entry 8b7a coloured by chain, top view.
Source organisms:
Primary publication:
Maytansinol Functionalization: Towards Useful Probes for Studying Microtubule Dynamics.
Boiarska Z, Pérez-Peña H, Abel AC, Marzullo P, Álvarez-Bernad B, Bonato F, Santini B, Horvath D, Lucena-Agell D, Vasile F, Sironi M, Díaz JF, Prota AE, Pieraccini S, Passarella D
Chemistry 29 e202203431 (2023)
PMID: 36468686

Function and Biology Details

Reaction catalysed: 
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-227043 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Tubulin--tyrosine ligase Chain: F

Ligands and Environments

8 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 6 x MG
Ligand CA 5 x CA
Ligand IMD 1 x IMD
Ligand GDP 2 x GDP
Ligand MES 1 x MES
Ligand Q0F 1 x Q0F
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 105.271Å b: 158.55Å c: 181.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.223
Expression system: Escherichia coli

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