› 8b8i

X-ray diffraction
2.75Å resolution

Nanobody (NbLumSyt1) bound to human Syt1

Released:
DOI: 10.2210/pdb8b8i/pdb
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PDB entry 8b8i coloured by chain, front view.
PDB entry 8b8i coloured by chain, side view.
PDB entry 8b8i coloured by chain, top view.
Source organisms:
Entry authors: Liu H, Scaletti E, Stenmark P

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273358 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nanobody (NbLumSyt1) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 118 amino acids
Theoretical weight: 12.62 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Synaptotagmin-1 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 60 amino acids
Theoretical weight: 6.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21579 (Residues: 1-60; Coverage: 14%)
Gene names: SVP65, SYT, SYT1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 87.955Å b: 100.226Å c: 103.939Å
α: 90° β: 100.832° γ: 90°
R-values:
R R work R free
0.265 0.263 0.298
Expression system: Escherichia coli

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