› 8b8j

Electron Microscopy
2.96Å resolution

Cryo-EM structure of Ca2+-bound mTMEM16F F518H mutant in Digitonin

Released:
DOI: 10.2210/pdb8b8j/pdb
PDB entry 8b8j coloured by chain, front view.
PDB entry 8b8j coloured by chain, side view.
PDB entry 8b8j coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the activation of the lipid scramblase TMEM16F.
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
OpenAccess logo Nat Commun 13 6692 (2022)
PMID: 36335104
Related structures: EMD-15914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180136 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anoctamin-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 975 amino acids
Theoretical weight: 113.45 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P9J9 (Residues: 1-911; Coverage: 100%)
Gene names: Ano6, Tmem16f
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-15914
Expression system: Homo sapiens

Quick links

Citations

2 review citations

In or out of the groove? Mechanisms of lipid scrambling by TMEM16 proteins.
Feng et al. (2024)
1 more

2 mentions without citation

Structural basis for the activation of the lipid scramblase TMEM16F.
Arndt et al. (2022)
1 more