› 8b9n

X-ray diffraction
2Å resolution

Crystal structure of NEI domain of mouse NEIL3 trapped in covalent complex with ssDNA with abasic site

Released:
DOI: 10.2210/pdb8b9n/pdb
PDB entry 8b9n coloured by chain, front view.
PDB entry 8b9n coloured by chain, side view.
PDB entry 8b9n coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Klima M, Boura E, Silhan J

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270316 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Endonuclease 8-like 3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 290 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8K203 (Residues: 1-282; Coverage: 47%)
Gene name: Neil3
Sequence domains: Formamidopyrimidine-DNA glycosylase H2TH domain
ssDNA with abasic site Chains: B, D
Molecule details ›
Chains: B, D
Length: 12 nucleotides
Theoretical weight: 3.51 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P4122
Unit cell:
a: 75.056Å b: 75.056Å c: 220.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links