› 8bav

X-ray diffraction
2.3Å resolution

Secretagogin (human) in complex with its target peptide from SNAP-25

Released:
DOI: 10.2210/pdb8bav/pdb
Model geometry
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PDB entry 8bav coloured by chain, front view.
PDB entry 8bav coloured by chain, side view.
PDB entry 8bav coloured by chain, top view.
Source organisms:
Primary publication:
A hydrophobic groove in secretagogin allows for alternate interactions with SNAP-25 and syntaxin-4 in endocrine tissues.
Szodorai E, Hevesi Z, Wagner L, Hökfelt TGM, Harkany T, Schnell R
Proc Natl Acad Sci U S A 121 e2309211121 (2024)
PMID: 38593081

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271870 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein; Synaptosomal-associated protein 25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 28.98 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-228, 232-233; Coverage: 96%)
  • Canonical: P60880 (Residues: 157-179; Coverage: 11%)
Gene names: GFP, SNAP, SNAP25
Sequence domains: Green fluorescent protein
Secretagogin Chains: C, D
Molecule details ›
Chains: C, D
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O76038 (Residues: 1-276; Coverage: 100%)
Gene names: SCGN, SECRET
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand CA 8 x CA
Ligand 144 1 x 144
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P212121
Unit cell:
a: 70.544Å b: 120.439Å c: 124.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.262
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

A hydrophobic groove in secretagogin allows for alternate interactions with SNAP-25 and syntaxin-4 in endocrine tissues.
Szodorai et al. (2024)

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