› 8bb1

Electron Microscopy
2.8Å resolution

T3 SAM lyase in complex with S-adenosylmethionine synthase

Released:
DOI: 10.2210/pdb8bb1/pdb
PDB entry 8bb1 coloured by chain, front view.
PDB entry 8bb1 coloured by chain, side view.
PDB entry 8bb1 coloured by chain, top view.
Source organisms:
Primary publication:
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase.
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
Cell Rep 42 112972 (2023)
PMID: 37578860
Related structures: EMD-15953

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227119 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 384 amino acids
Theoretical weight: 42 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A817 (Residues: 1-384; Coverage: 100%)
Gene names: JW2909, b2942, metK, metX
Sequence domains:
S-Adenosylmethionine lyase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 158 amino acids
Theoretical weight: 17.86 KDa
Source organism: Enterobacteria phage T3
Expression system: Escherichia coli
UniProt:
  • Canonical: P07693 (Residues: 1-152; Coverage: 100%)

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-15953
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

T5-like phage BF23 evades host-mediated DNA restriction and methylation.
Skutel et al. (2023)
1 more