› 8bc3

Electron Microscopy
2.1Å resolution

Cryo-EM Structure of a BmSF-TAL - Sulfofructose Schiff Base Complex

Released:
DOI: 10.2210/pdb8bc3/pdb
PDB entry 8bc3 coloured by chain, front view.
PDB entry 8bc3 coloured by chain, side view.
PDB entry 8bc3 coloured by chain, top view.
Source organism: Priestia aryabhattai
Primary publication:
Structure and mechanism of sulfofructose transaldolase, a key enzyme in sulfoquinovose metabolism.
Snow AJD, Sharma M, Abayakoon P, Williams SJ, Blaza JN, Davies GJ
Structure (2023)
PMID: 36805128
Related structures: EMD-15961

Function and Biology Details

Reaction catalysed: 
6-deoxy-6-sulfo-D-fructose + D-glyceraldehyde 3-phosphate = (2S)-3-sulfolactaldehyde + beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-deoxy-6-sulfo-D-fructose transaldolase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 226 amino acids
Theoretical weight: 25.31 KDa
Source organism: Priestia aryabhattai
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5E1T2 (Residues: 1-226; Coverage: 100%)
Gene names: BMQ_3648, sqvA
Sequence domains: Transaldolase/Fructose-6-phosphate aldolase

Ligands and Environments

1 bound ligand:
Ligand QC9 10 x QC9
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.1Å
Relevant EMDB volumes: EMD-15961
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Oxygenolytic sulfoquinovose degradation by an iron-dependent alkanesulfonate dioxygenase.
Ye et al. (2023)

1 mention without citation

Municipal wastewater monitoring revealed the predominance of bla GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla GES variant bla GES-48.
Tanabe et al. (2023)