› 8be2

X-ray diffraction
1.9Å resolution

Crystal structure of SOS1-Nanobody77

Released:
DOI: 10.2210/pdb8be2/pdb
PDB entry 8be2 coloured by chain, front view.
PDB entry 8be2 coloured by chain, side view.
PDB entry 8be2 coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric nanobodies to study the interactions between SOS1 and RAS.
Fischer B, Uchański T, Sheryazdanova A, Gonzalez S, Volkov AN, Brosens E, Zögg T, Kalichuk V, Ballet S, Versées W, Sablina AA, Pardon E, Wohlkönig A, Steyaert J
Nat Commun 15 6214 (2024)
PMID: 39043660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-555066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Son of sevenless homolog 1 Chain: S
Molecule details ›
Chain: S
Length: 507 amino acids
Theoretical weight: 59.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07889 (Residues: 564-1049; Coverage: 37%)
Gene name: SOS1
Sequence domains:
Nanobody 77 Chain: N
Molecule details ›
Chain: N
Length: 127 amino acids
Theoretical weight: 13.96 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 80.533Å b: 102.933Å c: 108.494Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.211
Expression system: Escherichia coli

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