› 8bel

Electron Microscopy
2.25Å resolution

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CIII membrane domain)

Released:
DOI: 10.2210/pdb8bel/pdb
PDB entry 8bel coloured by chain, front view.
PDB entry 8bel coloured by chain, side view.
PDB entry 8bel coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution.
Klusch N, Dreimann M, Senkler J, Rugen N, Kühlbrandt W, Braun HP
OpenAccess logo Nat Plants (2022)
PMID: 36585502
Related structures: EMD-16007

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-154884 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Cytochrome b Chains: C, M
Molecule details ›
Chains: C, M
Length: 393 amino acids
Theoretical weight: 44.31 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: P42792 (Residues: 1-393; Coverage: 100%)
Gene names: At2g07727, AtMg00220, COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome b-c1 complex subunit Rieske-1, mitochondrial Chains: D, N
Molecule details ›
Chains: D, N
Length: 272 amino acids
Theoretical weight: 29.65 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q94JS0 (Residues: 1-272; Coverage: 100%)
Gene names: At5g13430, T22N19.80, UCR1-1
Sequence domains:
Cytochrome c1 2, heme protein, mitochondrial Chains: E, O
Molecule details ›
Chains: E, O
Length: 307 amino acids
Theoretical weight: 33.73 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9FKS5 (Residues: 1-307; Coverage: 100%)
Gene names: At5g40810, CYC1-2, MHK7.4
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit 8-1, mitochondrial Chains: G, Q
Molecule details ›
Chains: G, Q
Length: 72 amino acids
Theoretical weight: 8.52 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9SG91 (Residues: 1-72; Coverage: 100%)
Gene names: At3g10860, T7M13.6, UCRQ-1
Sequence domains: Cytochrome b-c1 complex subunit 8
Cytochrome b-c1 complex subunit 6-1, mitochondrial Chains: H, R
Molecule details ›
Chains: H, R
Length: 69 amino acids
Theoretical weight: 8 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q0WWE3 (Residues: 1-69; Coverage: 100%)
Gene names: At1g15120, F9L1.5, QCR6-1
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 9, mitochondrial Chains: I, S
Molecule details ›
Chains: I, S
Length: 72 amino acids
Theoretical weight: 8.46 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9LXJ2 (Residues: 1-72; Coverage: 100%)
Gene names: At3g52730, F3C22.130, QCR9
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Cytochrome b-c1 complex subunit 10, mitochondrial Chains: J, T
Molecule details ›
Chains: J, T
Length: 57 amino acids
Theoretical weight: 5.98 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q94K78 (Residues: 1-57; Coverage: 100%)
Gene names: At2g40765, UCRY

Ligands and Environments

10 bound ligands:
Ligand HEM 6 x HEM
Ligand UQ5 3 x UQ5
Ligand 3PH 6 x 3PH
Ligand PGT 4 x PGT
Ligand CDL 7 x CDL
Ligand PC7 5 x PC7
Ligand FES 2 x FES
Ligand PTY 3 x PTY
Ligand UQ7 1 x UQ7
Ligand Q7G 2 x Q7G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.25Å
Relevant EMDB volumes: EMD-16007

Quick links

Citations

3 review citations

The biogenesis and regulation of the plant oxidative phosphorylation system.
Ghifari et al. (2023)
2 more