› 8bh1

Electron Microscopy
3.8Å resolution

Core divisome complex FtsWIQBL from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb8bh1/pdb
PDB entry 8bh1 coloured by chain, front view.
PDB entry 8bh1 coloured by chain, side view.
PDB entry 8bh1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL.
Käshammer L, van den Ent F, Jeffery M, Jean NL, Hale VL, Löwe J
OpenAccess logo Nat Microbiol (2023)
PMID: 37127704
Related structures: EMD-16042

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-216214 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Probable peptidoglycan glycosyltransferase FtsW Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 48.24 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HW00 (Residues: 1-399; Coverage: 100%)
Gene names: PA4413, ftsW
Sequence domains: Cell cycle protein
Peptidoglycan D,D-transpeptidase FtsI Chain: B
Molecule details ›
Chain: B
Length: 579 amino acids
Theoretical weight: 62.93 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD46 (Residues: 1-579; Coverage: 100%)
Gene names: PA4418, ftsI, pbpB
Sequence domains:
Cell division protein FtsQ Chain: C
Molecule details ›
Chain: C
Length: 287 amino acids
Theoretical weight: 32.29 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XDA7 (Residues: 1-287; Coverage: 100%)
Gene names: PA4409, ftsQ
Sequence domains:
Cell division protein FtsL Chain: D
Molecule details ›
Chain: D
Length: 97 amino acids
Theoretical weight: 11.15 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVZ6 (Residues: 1-97; Coverage: 100%)
Gene names: PA4419, ftsL
Sequence domains: Cell division protein FtsL
Cell division protein FtsB Chain: E
Molecule details ›
Chain: E
Length: 108 amino acids
Theoretical weight: 12.3 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXZ6 (Residues: 1-94; Coverage: 100%)
Gene names: PA3634, ftsB
Sequence domains: Septum formation initiator

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-16042
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Roles of ESCRT-III polymers in cell division across the tree of life.
Carlton et al. (2023)
2 more

1 mention without citation

Structural insights into the activation of the divisome complex FtsWIQLB.
Yang et al. (2024)