8bh7

Electron Microscopy
4.23Å resolution

The complex of immature 30S ribosomal subunit with Ribosome maturation factor P (RimP) from Staphylococcus aureus

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-272513 (preferred)
Entry contents:
20 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
Ribosome maturation factor RimP Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.72 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q2G2D3 (Residues: 2-155; Coverage: 99%)
Gene names: SAOUHSC_01242, rimP
Sequence domains:
Small ribosomal subunit protein uS2 Chain: b
Molecule details ›
Chain: b
Length: 255 amino acids
Theoretical weight: 29.14 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FZ25 (Residues: 1-255; Coverage: 100%)
Gene names: SAOUHSC_01232, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS4 Chain: d
Small ribosomal subunit protein uS3 Chain: c
Small ribosomal subunit protein uS5 Chain: e
Small ribosomal subunit protein bS6 Chain: f
Small ribosomal subunit protein uS7 Chain: g
Small ribosomal subunit protein uS8 Chain: h
Small ribosomal subunit protein uS11 Chain: k
Small ribosomal subunit protein uS12 Chain: l
Small ribosomal subunit protein uS9 Chain: i
Small ribosomal subunit protein uS10 Chain: j
Small ribosomal subunit protein uS13 Chain: m
Small ribosomal subunit protein uS14B Chain: n
Small ribosomal subunit protein uS19 Chain: s
Small ribosomal subunit protein uS15 Chain: o
Small ribosomal subunit protein bS16 Chain: p
Small ribosomal subunit protein uS17 Chain: q
Small ribosomal subunit protein bS18 Chain: r
Small ribosomal subunit protein bS20 Chain: t
16S ribosomal RNA Chain: a

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.23Å
Relevant EMDB volumes: EMD-16049
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'