› 8bih

X-ray diffraction
2.4Å resolution

O-Methyltransferase Plu4890 in complex with SAH and AQ-284b

Released:
DOI: 10.2210/pdb8bih/pdb
PDB entry 8bih coloured by chain, front view.
PDB entry 8bih coloured by chain, side view.
PDB entry 8bih coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.
Huber EM, Kreling L, Heinrich AK, Dünnebacke M, Pöthig A, Bode HB, Groll M
Structure (2023)
PMID: 36963398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 319 amino acids
Theoretical weight: 36.55 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY08 (Residues: 1-318; Coverage: 100%)
Gene name: plu4890
Sequence domains: O-methyltransferase domain

Ligands and Environments

5 bound ligands:
Ligand SAH 12 x SAH
Ligand QOT 9 x QOT
Ligand CL 4 x CL
Ligand GOL 2 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 91Å b: 91.13Å c: 150.41Å
α: 89.96° β: 106.18° γ: 119.97°
R-values:
R R work R free
0.269 0.267 0.296
Expression system: Escherichia coli BL21(DE3)

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