› 8bii

X-ray diffraction
2.8Å resolution

O-Methyltransferase Plu4895 (mutant H229N) in complex with SAH

Released:
DOI: 10.2210/pdb8bii/pdb
PDB entry 8bii coloured by chain, front view.
PDB entry 8bii coloured by chain, side view.
PDB entry 8bii coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.
Huber EM, Kreling L, Heinrich AK, Dünnebacke M, Pöthig A, Bode HB, Groll M
Structure (2023)
PMID: 36963398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 335 amino acids
Theoretical weight: 38.41 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY04 (Residues: 1-317; Coverage: 100%)
Gene name: plu4895
Sequence domains: O-methyltransferase domain

Ligands and Environments

2 bound ligands:
Ligand SAH 8 x SAH
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 58.92Å b: 98.3Å c: 531.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.281
Expression system: Escherichia coli BL21(DE3)

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Citations

4 mentions without citation

Recent advances in musculoskeletal local drug delivery.
Zhang et al. (2019)
3 more