› 8bij

X-ray diffraction
1.55Å resolution

O-Methyltransferase Plu4894 (mutant I88M, W91L, C97Y, S142L, G146V, Y258M, L270F, S309Y) in complex with SAH

Released:
DOI: 10.2210/pdb8bij/pdb
PDB entry 8bij coloured by chain, front view.
PDB entry 8bij coloured by chain, side view.
PDB entry 8bij coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.
Huber EM, Kreling L, Heinrich AK, Dünnebacke M, Pöthig A, Bode HB, Groll M
Structure (2023)
PMID: 36963398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 36.19 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY05 (Residues: 1-317; Coverage: 100%)
Gene name: plu4894
Sequence domains: O-methyltransferase domain

Ligands and Environments

4 bound ligands:
Ligand SAH 2 x SAH
Ligand EDO 3 x EDO
Ligand NA 2 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 72.31Å b: 76.46Å c: 113.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Identifying people with a learning disability: an advanced search for general practice.
Russell et al. (2017)