› 8bk8

Electron Microscopy
2.9Å resolution

Cryo-EM structure of beta-galactosidase at 2.9 A resolution plunged 205 ms after mixing with apoferritin

Released:
DOI: 10.2210/pdb8bk8/pdb
PDB entry 8bk8 coloured by chain, front view.
PDB entry 8bk8 coloured by chain, side view.
PDB entry 8bk8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting.
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023)
PMID: 37592181
Related structures: EMD-16092

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1031 amino acids
Theoretical weight: 117.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 1-1024; Coverage: 100%)
Gene names: JW0335, b0344, lacZ
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-16092
Expression system: Escherichia coli

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Citations

1 review citation

Na+ Binding and Transport: Insights from Light-Driven Na+-Pumping Rhodopsin.
Yang et al. (2023)