› 8bkf

X-ray diffraction
1.22Å resolution

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (crystal M200T#o)

Released:
DOI: 10.2210/pdb8bkf/pdb
PDB entry 8bkf coloured by chain, front view.
PDB entry 8bkf coloured by chain, side view.
PDB entry 8bkf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The effects of nature-inspired amino acid substitutions on structural and biochemical properties of the E. coli L-asparaginase EcAIII.
Janicki M, Ściuk A, Zielezinski A, Ruszkowski M, Ludwików A, Karlowski WM, Jaskolski M, Loch JI
OpenAccess logo Protein Sci e4647 (2023)
PMID: 37095066

Function and Biology Details

Reaction catalysed: 
Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Isoaspartyl peptidase subunit alpha Chains: AAA, CCC
Molecule details ›
Chains: AAA, CCC
Length: 178 amino acids
Theoretical weight: 19.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37595 (Residues: 1-178; Coverage: 56%)
Gene names: JW0812, b0828, iaaA, spt, ybiK
Sequence domains: Asparaginase
Isoaspartyl peptidase subunit beta Chains: BBB, DDD
Molecule details ›
Chains: BBB, DDD
Length: 143 amino acids
Theoretical weight: 14.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37595 (Residues: 179-321; Coverage: 45%)
Gene names: JW0812, b0828, iaaA, spt, ybiK
Sequence domains: Asparaginase

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand CL 4 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 49.852Å b: 75.886Å c: 147.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.15
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

The effects of nature-inspired amino acid substitutions on structural and biochemical properties of the E. coli L-asparaginase EcAIII.
Janicki et al. (2023)