› 8blc

Electron Microscopy
2.7Å resolution

Structure of the GroEL-ATP complex plunge-frozen 50 ms after mixing with ATP

Released:
DOI: 10.2210/pdb8blc/pdb
PDB entry 8blc coloured by chain, front view.
PDB entry 8blc coloured by chain, side view.
PDB entry 8blc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting.
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023)
PMID: 37592181
Related structures: EMD-16106

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 548 amino acids
Theoretical weight: 57.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F5 (Residues: 1-548; Coverage: 100%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

3 bound ligands:
Ligand MG 14 x MG
Ligand ATP 14 x ATP
Ligand K 14 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-16106
Expression system: Escherichia coli

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Citations

1 review citation

Na+ Binding and Transport: Insights from Light-Driven Na+-Pumping Rhodopsin.
Yang et al. (2023)