› 8bms

Electron Microscopy
2.6Å resolution

Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation

Released:
DOI: 10.2210/pdb8bms/pdb
PDB entry 8bms coloured by chain, front view.
PDB entry 8bms coloured by chain, side view.
PDB entry 8bms coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Primary publication:
Expulsion mechanism of the substrate-translocating subunit in ECF transporters.
Thangaratnarajah C, Nijland M, Borges-Araújo L, Jeucken A, Rheinberger J, Marrink SJ, Souza PCT, Paulino C, Slotboom DJ
OpenAccess logo Nat Commun 14 4484 (2023)
PMID: 37491368
Related structures: EMD-16124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-557248 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA1 Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 30.8 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBJ0 (Residues: 2-282; Coverage: 100%)
Gene names: Ldb0424, cbiO1, ecfA1
Sequence domains: ABC transporter
Energy-coupling factor transporter ATP-binding protein EcfA2 Chain: B
Molecule details ›
Chain: B
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBI9 (Residues: 1-287; Coverage: 100%)
Gene names: Ldb0425, cbiO2, ecfA2
Sequence domains: ABC transporter
Energy-coupling factor transporter transmembrane protein EcfT Chain: C
Molecule details ›
Chain: C
Length: 265 amino acids
Theoretical weight: 30.29 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBI8 (Residues: 1-265; Coverage: 100%)
Gene names: Ldb0426, cbiQ, ecfT
Sequence domains: Cobalt transport protein

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-16124
Expression system: Escherichia coli MC1061

Quick links

Citations

1 citations in other articles

The S-component fold: a link between bacterial transporters and receptors.
Partipilo et al. (2024)