› 8bnx

X-ray diffraction
3.12Å resolution

Crystal structure of Pif1 from Deferribacter desulfuricans in complex with AMPPNP

Released:
DOI: 10.2210/pdb8bnx/pdb
PDB entry 8bnx coloured by chain, front view.
PDB entry 8bnx coloured by chain, side view.
PDB entry 8bnx coloured by chain, top view.
Source organism: Deferribacter desulfuricans
Primary publication:
Structural Studies of Pif1 Helicases from Thermophilic Bacteria.
Réty S, Zhang Y, Fu W, Wang S, Chen WF, Xi XG
OpenAccess logo Microorganisms 11 (2023)
PMID: 36838444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA family ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 510 amino acids
Theoretical weight: 58.77 KDa
Source organism: Deferribacter desulfuricans
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: D3PAZ1 (Residues: 1-510; Coverage: 100%)
Gene name: DEFDS_0256
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21212
Unit cell:
a: 143.797Å b: 151.376Å c: 51.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.255
Expression system: Escherichia coli K-12

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