› 8bo2

Electron Microscopy
3.1Å resolution

BAM-EspP complex structure with BamA-S425C/EspP-S1299C mutations in nanodisc

Released:
DOI: 10.2210/pdb8bo2/pdb
PDB entry 8bo2 coloured by chain, front view.
PDB entry 8bo2 coloured by chain, side view.
PDB entry 8bo2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis of BAM-mediated outer membrane β-barrel protein assembly.
Shen C, Chang S, Luo Q, Chan KC, Zhang Z, Luo B, Xie T, Lu G, Zhu X, Wei X, Dong C, Zhou R, Zhang X, Tang X, Dong H
Nature 617 185-193 (2023)
PMID: 37100902
Related structures: EMD-16138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-269968 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 787 amino acids
Theoretical weight: 88.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: B1XD46 (Residues: 24-810; Coverage: 100%)
Gene names: ECDH10B_0157, bamA, yaeT
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 392 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Autotransporter protein EspP translocator Chain: P
Molecule details ›
Chain: P
Length: 353 amino acids
Theoretical weight: 38.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7BSW5 (Residues: 948-1300; Coverage: 28%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Autotransporter beta-domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-16138
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

Biogenesis of mitochondrial β-barrel membrane proteins.
Ganesan et al. (2024)
2 more