› 8bos

X-ray diffraction
2.1Å resolution

Transition state analogue complex of small G protein and its GAP effector

Released:
DOI: 10.2210/pdb8bos/pdb
PDB entry 8bos coloured by chain, front view.
PDB entry 8bos coloured by chain, side view.
PDB entry 8bos coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Baumann P, Jin Y

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133959 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Ras GTPase-activating protein 1 Chain: G
Molecule details ›
Chain: G
Length: 331 amino acids
Theoretical weight: 37.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20936 (Residues: 713-1042; Coverage: 32%)
Gene names: GAP, RASA, RASA1
Sequence domains: GTPase-activator protein for Ras-like GTPase

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand MGF 1 x MGF
Ligand MG 1 x MG
Ligand GAI 1 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 71.832Å b: 41.558Å c: 89.674Å
α: 90° β: 109.25° γ: 90°
R-values:
R R work R free
0.202 0.198 0.28
Expression system: Escherichia coli BL21(DE3)

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