› 8btp

Electron Microscopy
2.75Å resolution

Helical structure of BcThsA in complex with 1''-3'gc(etheno)ADPR

Released:
DOI: 10.2210/pdb8btp/pdb
PDB entry 8btp coloured by chain, front view.
PDB entry 8btp coloured by chain, side view.
PDB entry 8btp coloured by chain, top view.
Source organism: Bacillus cereus MSX-D12
Primary publication:
Activation of Thoeris antiviral system via SIR2 effector filament assembly.
Tamulaitiene G, Sabonis D, Sasnauskas G, Ruksenaite A, Silanskas A, Avraham C, Ofir G, Sorek R, Zaremba M, Siksnys V
Nature (2024)
PMID: 38383786
Related structures: EMD-16234

Function and Biology Details

Reaction catalysed: 
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+) hydrolase ThsA Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 479 amino acids
Theoretical weight: 55.42 KDa
Source organism: Bacillus cereus MSX-D12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J8G6Z1 (Residues: 1-476; Coverage: 100%)
Gene names: II9_05448, thsA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand RK3 12 x RK3
Ligand ENA 12 x ENA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.75Å
Relevant EMDB volumes: EMD-16234
Expression system: Escherichia coli BL21(DE3)

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