Function and Biology Details
Reactions catalysed:
ATP + H(2)O = ADP + phosphate
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero 42-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236732 (preferred)
Entry contents:
36 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (38 distinct):
General transcription and DNA repair factor IIH helicase/translocase subunit XPB
Chain: 0
Molecule details ›
Chain: 0
Length: 772 amino acids
Theoretical weight: 88.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 772 amino acids
Theoretical weight: 88.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P19447 (Residues: 1-772; Coverage: 99%)
Sequence domains:
General transcription and DNA repair factor IIH helicase subunit XPD
Chain: 1
Molecule details ›
Chain: 1
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P18074 (Residues: 1-760; Coverage: 100%)
Sequence domains:
General transcription factor IIH subunit 1
Chain: 2
Molecule details ›
Chain: 2
Length: 548 amino acids
Theoretical weight: 62.12 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 548 amino acids
Theoretical weight: 62.12 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P32780 (Residues: 1-548; Coverage: 100%)
Sequence domains:
General transcription factor IIH subunit 4
Chain: 3
Molecule details ›
Chain: 3
Length: 462 amino acids
Theoretical weight: 52.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 462 amino acids
Theoretical weight: 52.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q92759 (Residues: 1-462; Coverage: 100%)
Sequence domains:
General transcription factor IIH subunit 2
Chain: 4
Molecule details ›
Chain: 4
Length: 395 amino acids
Theoretical weight: 44.48 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 395 amino acids
Theoretical weight: 44.48 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q13888 (Residues: 1-395; Coverage: 100%)
Sequence domains:
General transcription factor IIH subunit 3
Chain: 5
Molecule details ›
Chain: 5
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Transcription factor Tfb4
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q13889 (Residues: 1-308; Coverage: 100%)
Sequence domains: Transcription factor Tfb4
General transcription factor IIH subunit 5
Chain: 6
Molecule details ›
Chain: 6
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
CDK-activating kinase assembly factor MAT1
Chain: 7
Molecule details ›
Cyclin-dependent kinase 7
Chain: 8
Molecule details ›
Chain: 8
Length: 346 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Protein kinase domain
Length: 346 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P50613 (Residues: 1-346; Coverage: 100%)
Sequence domains: Protein kinase domain
Cyclin-H
Chain: 9
Molecule details ›
Chain: 9
Length: 323 amino acids
Theoretical weight: 37.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 323 amino acids
Theoretical weight: 37.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P51946 (Residues: 1-323; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit
Chain: A
Molecule details ›
Chain: A
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa
UniProt:
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A7M4DUC2 (Residues: 1-1984; Coverage: 99%)
DNA-directed RNA polymerase subunit beta
Chain: B
Molecule details ›
Chain: B
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
I3LGP4 (Residues: 127-1300; Coverage: 90%)
Sequence domains:
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein
Chain: C
Molecule details ›
Chain: C
Length: 275 amino acids
Theoretical weight: 31.44 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 275 amino acids
Theoretical weight: 31.44 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
I3LCH3 (Residues: 1-275; Coverage: 100%)
Sequence domains:
RNA polymerase Rpb4/RPC9 core domain-containing protein
Chain: D
Molecule details ›
Chain: D
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb4
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A287ADR4 (Residues: 43-184; Coverage: 77%)
Sequence domains: RNA polymerase Rpb4
DNA-directed RNA polymerase II subunit E
Chain: E
Molecule details ›
Chain: E
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
I3LSI7 (Residues: 1-210; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A4X1VEK9 (Residues: 1-127; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit
Chain: G
Molecule details ›
Chain: G
Length: 172 amino acids
Theoretical weight: 19.31 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 172 amino acids
Theoretical weight: 19.31 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A4X1VKG7 (Residues: 1-172; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb8
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
I3LCB2 (Residues: 1-150; Coverage: 100%)
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase II subunit RPB9
Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 14.54 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 125 amino acids
Theoretical weight: 14.54 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
P60899 (Residues: 1-125; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Chain: J
Molecule details ›
Chain: J
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerases N / 8 kDa subunit
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A4X1VYD0 (Residues: 1-67; Coverage: 100%)
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein
Chain: K
Molecule details ›
Chain: K
Length: 117 amino acids
Theoretical weight: 13.31 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Length: 117 amino acids
Theoretical weight: 13.31 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
F1RKE4 (Residues: 1-117; Coverage: 100%)
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Chain: L
Molecule details ›
Chain: L
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Sus scrofa
UniProt:
- Canonical:
A0A4X1TRS6 (Residues: 1-58; Coverage: 100%)
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Transcription initiation factor IIB
Chain: M
Molecule details ›
Chain: M
Length: 316 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 316 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q00403 (Residues: 1-316; Coverage: 100%)
Sequence domains:
TATA-box-binding protein
Chain: O
Molecule details ›
Chain: O
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P20226 (Residues: 1-339; Coverage: 100%)
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
General transcription factor IIF subunit 1
Chain: Q
Molecule details ›
Chain: Q
Length: 517 amino acids
Theoretical weight: 58.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Length: 517 amino acids
Theoretical weight: 58.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P35269 (Residues: 1-517; Coverage: 100%)
Sequence domains: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
General transcription factor IIF subunit 2
Chain: R
Molecule details ›
Chain: R
Length: 249 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 249 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P13984 (Residues: 1-249; Coverage: 100%)
Sequence domains:
Transcription initiation factor IIA subunit 1
Chain: U
Molecule details ›
Chain: U
Length: 376 amino acids
Theoretical weight: 41.54 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Transcription factor IIA, alpha/beta subunit
Length: 376 amino acids
Theoretical weight: 41.54 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P52655 (Residues: 1-376; Coverage: 100%)
Sequence domains: Transcription factor IIA, alpha/beta subunit
Transcription initiation factor IIA subunit 2
Chain: V
Molecule details ›
Chain: V
Length: 109 amino acids
Theoretical weight: 12.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 109 amino acids
Theoretical weight: 12.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
P52657 (Residues: 1-109; Coverage: 100%)
Sequence domains:
General transcription factor IIE subunit 1
Chain: W
Molecule details ›
Chain: W
Length: 439 amino acids
Theoretical weight: 49.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 439 amino acids
Theoretical weight: 49.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P29083 (Residues: 1-439; Coverage: 100%)
Sequence domains:
Transcription initiation factor IIE subunit beta
Chain: X
Molecule details ›
Chain: X
Length: 291 amino acids
Theoretical weight: 33.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 291 amino acids
Theoretical weight: 33.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P29084 (Residues: 1-291; Coverage: 100%)
Sequence domains:
Unassigned peptide, likely XPB
Chain: Y
Molecule details ›
Chain: Y
Length: 8 amino acids
Theoretical weight: 699 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Length: 8 amino acids
Theoretical weight: 699 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Unassigned peptide, likely TFIIE-beta
Chain: Z
Molecule details ›
Chain: Z
Length: 19 amino acids
Theoretical weight: 1.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Length: 19 amino acids
Theoretical weight: 1.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Histone H3.2
Chains: a, e
Molecule details ›
Chains: a, e
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
- Canonical:
P84233 (Residues: 1-136; Coverage: 100%)
Histone H4
Chains: b, f
Molecule details ›
Chains: b, f
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
- Canonical:
P62799 (Residues: 1-103; Coverage: 100%)
Histone H2A type 1
Chains: c, g
Molecule details ›
Chains: c, g
Length: 130 amino acids
Theoretical weight: 14.09 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
Length: 130 amino acids
Theoretical weight: 14.09 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
- Canonical:
P06897 (Residues: 1-130; Coverage: 100%)
Histone H2B 1.1
Chains: d, h
Molecule details ›
Chains: d, h
Length: 126 amino acids
Theoretical weight: 13.97 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
Length: 126 amino acids
Theoretical weight: 13.97 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
- Canonical:
P02281 (Residues: 1-126; Coverage: 100%)
Non-template DNA
Chain: N
Molecule details ›
Chain: N
Length: 218 nucleotides
Theoretical weight: 67.22 KDa
Length: 218 nucleotides
Theoretical weight: 67.22 KDa
Template DNA
Chain: T
Molecule details ›
Chain: T
Length: 218 nucleotides
Theoretical weight: 67.41 KDa
Length: 218 nucleotides
Theoretical weight: 67.41 KDa