› 8bw9

Electron Microscopy
3.32Å resolution

Cryo-EM structure of the RAF activating complex KSR-MEK-CNK-HYP

Released:
DOI: 10.2210/pdb8bw9/pdb
PDB entry 8bw9 coloured by chain, front view.
PDB entry 8bw9 coloured by chain, side view.
PDB entry 8bw9 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The CNK-HYP scaffolding complex promotes RAF activation by enhancing KSR-MEK interaction.
Maisonneuve P, Sahmi M, Bergeron-Labrecque F, Ma XI, Queguiner J, Arseneault G, Lefrançois M, Kurinov I, Fronzes R, Sicheri F, Therrien M
OpenAccess logo Nat Struct Mol Biol (2024)
PMID: 38388830
Related structures: EMD-16281

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-244254 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein aveugle Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 13.14 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ML92 (Residues: 2-106; Coverage: 99%)
Gene names: CG30476, ave
Sequence domains: SAM domain (Sterile alpha motif)
SD09985p Chain: B
Molecule details ›
Chain: B
Length: 335 amino acids
Theoretical weight: 38.53 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7KNQ9 (Residues: 1-330; Coverage: 21%)
Gene names: CG6556, CNK, Cnk, Dmel\CG6556, Dmel_CG6556, EC2-3, EK2-3, anon-WO0140519.129, anon-WO0257455.27, cnk, l(2)k16314, sag
Sequence domains:
Dual specificity mitogen-activated protein kinase kinase dSOR1 Chain: C
Molecule details ›
Chain: C
Length: 396 amino acids
Theoretical weight: 43.93 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q24324 (Residues: 1-396; Coverage: 100%)
Gene names: CG15793, Dsor1
Sequence domains: Protein kinase domain
Kinase suppressor of ras, isoform D Chain: D
Molecule details ›
Chain: D
Length: 316 amino acids
Theoretical weight: 36.47 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q24171 (Residues: 654-966; Coverage: 32%)
Gene names: CG2899, Dmel\CG2899, Dmel_CG2899, EC3-1, EK3-1, KSR, KSR-1, Ksr, Ksr-1, SR3-1, dKsr, ksr, l(3)j5E2
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand QOM 1 x QOM
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.32Å
Relevant EMDB volumes: EMD-16281
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

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