› 8bwc

Electron Microscopy
3.5Å resolution

E. coli BAM complex (BamABCDE) wild-type

Released:
DOI: 10.2210/pdb8bwc/pdb
PDB entry 8bwc coloured by chain, front view.
PDB entry 8bwc coloured by chain, side view.
PDB entry 8bwc coloured by chain, top view.
Source organisms:
Primary publication:
Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells.
Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Böhringer N, Schäberle TF, Ranson NA, Radford SE, Pliotas C
Angew Chem Int Ed Engl e202218783 (2023)
PMID: 37162386
Related structures: EMD-16282

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-141767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 790 amino acids
Theoretical weight: 88.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 21-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 373 amino acids
Theoretical weight: 39.88 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P77774 (Residues: 20-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A903 (Residues: 25-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 25.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC02 (Residues: 20-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 104 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A937 (Residues: 20-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-16282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 citation in other articles

Drug Binding to BamA Targets Its Lateral Gate.
Kuo et al. (2023)
1 more