› 8bwl

X-ray diffraction
1.96Å resolution

Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1) in complex with human Growth Differentiation factor 5 (GDF5) and calcium

Released:
DOI: 10.2210/pdb8bwl/pdb
PDB entry 8bwl coloured by chain, front view.
PDB entry 8bwl coloured by chain, side view.
PDB entry 8bwl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of BMP signal control by Twisted gastrulation.
Malinauskas T, Moore G, Rudolf AF, Eggington H, Belnoue-Davis HL, El Omari K, Griffiths SC, Woolley RE, Duman R, Wagner A, Leedham SJ, Baldock C, Ashe HL, Siebold C
Nat Commun 15 4976 (2024)
PMID: 38862520

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246050 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth/differentiation factor 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 13.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43026 (Residues: 382-501; Coverage: 25%)
Gene names: BMP14, CDMP1, GDF5
Sequence domains: Transforming growth factor beta like domain
Twisted gastrulation protein homolog 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 69 amino acids
Theoretical weight: 7.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9GZX9 (Residues: 26-83; Coverage: 29%)
Gene names: PSEC0250, TSG, TWSG1

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 57.047Å b: 88.588Å c: 97.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

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