› 8bxx

X-ray diffraction
1.97Å resolution

Crystal structure of formate dehydrogenase FDH2 enzyme from Granulicella mallensis MP5ACTX8 in complex with NAD and azide.

Released:
DOI: 10.2210/pdb8bxx/pdb
PDB entry 8bxx coloured by chain, front view.
PDB entry 8bxx coloured by chain, side view.
PDB entry 8bxx coloured by chain, top view.
Source organism: Granulicella mallensis MP5ACTX8
Primary publication:
From the amelioration of a NADP+-dependent formate dehydrogenase to the discovery of a new enzyme: round trip from theory to practice
Rubini R, Robescu MS, Tavanti M, Beneventi E, Lonigro C, Niero M, Zito F, Filippini F, Cendron L, Bergantino E
ChemCatChem (2020)

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: AA, BB, CC, DD
Molecule details ›
Chains: AA, BB, CC, DD
Length: 386 amino acids
Theoretical weight: 42.59 KDa
Source organism: Granulicella mallensis MP5ACTX8
Expression system: Escherichia coli
UniProt:
  • Canonical: G8NTI5 (Residues: 1-386; Coverage: 100%)
Gene name: AciX8_0868
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NAD 4 x NAD
Ligand AZI 4 x AZI
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 72.066Å b: 128.81Å c: 85.664Å
α: 90° β: 100.4° γ: 90°
R-values:
R R work R free
0.188 0.188 0.234
Expression system: Escherichia coli

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