› 8byl

Electron Microscopy
3.5Å resolution

Cryo-EM structure of SKP1-SKP2-CKS1 from the SCFSKP2 E3 ligase complex

Released:
DOI: 10.2210/pdb8byl/pdb
PDB entry 8byl coloured by chain, front view.
PDB entry 8byl coloured by chain, side view.
PDB entry 8byl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of SKP1-SKP2-CKS1 in complex with CDK2-cyclin A-p27KIP1.
Rowland RJ, Heath R, Maskell D, Thompson RF, Ranson NA, Blaza JN, Endicott JA, Noble MEM, Salamina M
OpenAccess logo Sci Rep 13 10718 (2023)
PMID: 37400515
Related structures: EMD-16327

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-155477 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 100%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
S-phase kinase-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 424 amino acids
Theoretical weight: 47.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13309 (Residues: 1-424; Coverage: 100%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Cyclin-dependent kinases regulatory subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 79 amino acids
Theoretical weight: 9.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61024 (Residues: 1-79; Coverage: 100%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Cyclin-dependent kinase inhibitor 1B Chain: D
Molecule details ›
Chain: D
Length: 198 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46527 (Residues: 1-198; Coverage: 100%)
Gene names: CDKN1B, KIP1, p27
Sequence domains: Cyclin-dependent kinase inhibitor

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-16327
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A sporadic Parkinson's disease model via silencing of the ubiquitin-proteasome/E3 ligase component, SKP1A.
Fishman-Jacob et al. (2023)
1 other article

1 mention without citation

Cryo-EM structure of SKP1-SKP2-CKS1 in complex with CDK2-cyclin A-p27KIP1.
Rowland et al. (2023)