8bz1

Electron Microscopy
3.8Å resolution

RNA polymerase II core pre-initiation complex with the proximal +1 nucleosome (cPIC-Nuc10W)

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
PDBe Complex ID:
PDB-CPX-269054 (preferred)
Entry contents:
22 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (24 distinct):
DNA-directed RNA polymerase subunit Chain: A
Molecule details ›
Chain: A
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein Chain: C
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: D
DNA-directed RNA polymerase II subunit E Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: K
RNA polymerase II, I and III subunit K Chain: L
Transcription initiation factor IIB Chain: M
TATA-box-binding protein Chain: O
General transcription factor IIF subunit 1 Chain: Q
General transcription factor IIF subunit 2 Chain: R
Transcription initiation factor IIA subunit 1 Chain: U
Transcription initiation factor IIA subunit 2 Chain: V
Histone H3.2 Chains: a, e
Histone H4 Chains: b, f
Histone H2A type 1 Chains: c, g
Histone H2B 1.1 Chains: d, h
Non-template DNA Chain: N
Template DNA Chain: T

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-16335
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Not provided