› 8c0n

X-ray diffraction
2.9Å resolution

Crystal structure of the red form of the mTagFT fluorescent timer

Released:
DOI: 10.2210/pdb8c0n/pdb
PDB entry 8c0n coloured by chain, front view.
PDB entry 8c0n coloured by chain, side view.
PDB entry 8c0n coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Blue-to-Red TagFT, mTagFT, mTsFT, and Green-to-FarRed mNeptusFT2 Proteins, Genetically Encoded True and Tandem Fluorescent Timers.
Subach OM, Vlaskina AV, Agapova YK, Nikolaeva AY, Anokhin KV, Piatkevich KD, Patrushev MV, Boyko KM, Subach FV
OpenAccess logo Int J Mol Sci 24 (2023)
PMID: 36834686

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP611 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 31.23 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ISF8 (Residues: 4-231; Coverage: 97%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRP 4 x NRP

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P2
Unit cell:
a: 60.645Å b: 95.317Å c: 95.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.277
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Combined Structural and Computational Study of the mRubyFT Fluorescent Timer Locked in Its Blue Form.
Boyko et al. (2023)