› 8c10

X-ray diffraction
1Å resolution

Biochemical and structural characterisation of an alkaline family GH5 cellulase from a shipworm symbiont

Released:
DOI: 10.2210/pdb8c10/pdb
PDB entry 8c10 coloured by chain, front view.
PDB entry 8c10 coloured by chain, side view.
PDB entry 8c10 coloured by chain, top view.
Source organism: Teredinibacter waterburyi
Primary publication:
Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara.
Junghare M, Manavalan T, Fredriksen L, Leiros I, Altermark B, Eijsink VGH, Vaaje-Kolstad G
OpenAccess logo Biotechnol Biofuels Bioprod 16 61 (2023)
PMID: 37016457

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH5 Cellulase Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.28 KDa
Source organism: Teredinibacter waterburyi
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand TRS 1 x TRS
Ligand EDO 2 x EDO
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 45.041Å b: 68.426Å c: 87.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.112 0.133
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 mentions without citation

In vitro generation and bioactivity evaluation of C-reactive protein intermediate.
Lv et al. (2018)
1 more