› 8c34

X-ray diffraction
1.82Å resolution

Aerobic light exposed 1.8 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH

Released:
DOI: 10.2210/pdb8c34/pdb
PDB entry 8c34 coloured by chain, front view.
PDB entry 8c34 coloured by chain, side view.
PDB entry 8c34 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Redox driven B12-ligand switch drives CarH photoresponse.
Poddar H, Rios-Santacruz R, Heyes DJ, Shanmugam M, Brookfield A, Johannissen LO, Levy CW, Jeffreys LN, Zhang S, Sakuma M, Colletier JP, Hay S, Schirò G, Weik M, Scrutton NS, Leys D
OpenAccess logo Nat Commun 14 5082 (2023)
PMID: 37604813

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable transcriptional regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 78-285; Coverage: 73%)
Gene name: TT_P0056
Sequence domains: B12 binding domain

Ligands and Environments

3 bound ligands:
Ligand B12 4 x B12
Ligand PEG 1 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 60.87Å b: 72.69Å c: 201.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.198
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Redox driven B12-ligand switch drives CarH photoresponse.
Poddar et al. (2023)