8c3v

X-ray diffraction
2.74Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-272858 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
BA.2-13 heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 231 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
BA.2-13 light chain Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 213 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Spike protein S1 Chains: R, X, Y
Molecule details ›
Chains: R, X, Y
Length: 202 amino acids
Theoretical weight: 22.94 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 333-528; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
Nanobody C1 Chains: I, J, K
Molecule details ›
Chains: I, J, K
Length: 131 amino acids
Theoretical weight: 14.66 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 105.925Å b: 160.817Å c: 172.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.258
Expression systems:
  • Homo sapiens
  • Escherichia coli