› 8c4n

X-ray diffraction
1.75Å resolution

CdaA-AMP complex

Released:
DOI: 10.2210/pdb8c4n/pdb
PDB entry 8c4n coloured by chain, front view.
PDB entry 8c4n coloured by chain, side view.
PDB entry 8c4n coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Computer-aided design of a cyclic di-AMP synthesizing enzyme CdaA inhibitor.
Neumann P, Kloskowski P, Ficner R
OpenAccess logo Microlife 4 uqad021 (2023)
PMID: 37223749

Function and Biology Details

Reaction catalysed: 
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diadenylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 19.37 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y5E4 (Residues: 101-273; Coverage: 63%)
Gene names: cdaA, dacA, lmo2120
Sequence domains: DisA bacterial checkpoint controller nucleotide-binding

Ligands and Environments

3 bound ligands:
Ligand AMP 2 x AMP
Ligand NA 1 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 41.61Å b: 64.72Å c: 129.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.255
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The many roles of cyclic di-AMP to control the physiology of Bacillus subtilis.
Herzberg et al. (2023)
2 other articles