› 8c5c

Electron Microscopy
5.3Å resolution

microtubule decorated with tubulin oligomers in presence of APC C-terminal domain. (here only map corresponding to the 13-pf microtubule is represented)

Released:
DOI: 10.2210/pdb8c5c/pdb
PDB entry 8c5c coloured by chain, front view.
PDB entry 8c5c coloured by chain, side view.
PDB entry 8c5c coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The mitotic role of Adenomatous Polyposis Coli requires its bilateral interaction with tubulin and microtubules.
Serre L, Delaroche J, Vinit A, Schoehn G, Denarier E, Fourest-Lieuvin A, Arnal I
J Cell Sci (2022)
PMID: 36541084
Related structures: EMD-16435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105073 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin beta 4B class IVb Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y, z
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y, z
Length: 446 amino acids
Theoretical weight: 50.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: A0A452DIL8 (Residues: 1-19; Coverage: 23%)
Gene name: TUBB4B
Tubulin alpha-1B chain Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand TA1 13 x TA1
Ligand GDP 13 x GDP
Ligand MG 13 x MG
Ligand GTP 13 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.3Å
Relevant EMDB volumes: EMD-16435

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