› 8c76

X-ray diffraction
2.5Å resolution

Light-state 2.5 Angstrom wild-type X-ray crystal structure of the cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions from a form 2 crystal illuminated during 5 s

Released:
DOI: 10.2210/pdb8c76/pdb
PDB entry 8c76 coloured by chain, front view.
PDB entry 8c76 coloured by chain, side view.
PDB entry 8c76 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Redox driven B12-ligand switch drives CarH photoresponse.
Poddar H, Rios-Santacruz R, Heyes DJ, Shanmugam M, Brookfield A, Johannissen LO, Levy CW, Jeffreys LN, Zhang S, Sakuma M, Colletier JP, Hay S, Schirò G, Weik M, Scrutton NS, Leys D
OpenAccess logo Nat Commun 14 5082 (2023)
PMID: 37604813

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor CarH Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53W62 (Residues: 78-285; Coverage: 73%)
Gene names: TTHB100, carH
Sequence domains: B12 binding domain

Ligands and Environments

1 bound ligand:
Ligand B12 4 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 61.856Å b: 73.157Å c: 204.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.235
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Redox driven B12-ligand switch drives CarH photoresponse.
Poddar et al. (2023)