› 8c7p

X-ray diffraction
3Å resolution

Tagless BtuM in complex with cyanocobalamin

Released:
DOI: 10.2210/pdb8c7p/pdb
PDB entry 8c7p coloured by chain, front view.
PDB entry 8c7p coloured by chain, side view.
PDB entry 8c7p coloured by chain, top view.
Source organism: Thiobacillus denitrificans ATCC 25259
Primary publication:
Cobalamin decyanation by the membrane transporter BtuM.
Martínez Felices JM, Barreto YB, Thangaratnarajah C, Whittaker JJ, Alencar AM, Guskov A, Slotboom DJ
Structure 32 1165-1173.e3 (2024)
PMID: 38733996

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin ABC transporter Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.79 KDa
Source organism: Thiobacillus denitrificans ATCC 25259
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q3SFD8 (Residues: 2-198; Coverage: 100%)
Gene name: Tbd_2719

Ligands and Environments

1 bound ligand:
Ligand B12 1 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3221
Unit cell:
a: 91.318Å b: 91.318Å c: 77.391Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.225 0.263
Expression system: Escherichia coli MC1061

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