› 8c7s

X-ray diffraction
3.05Å resolution

Transcriptional pleiotropic repressor CodY from Staphylococcus aureus in complex with Ile, GTP, and a 30-bp DNA fragment encompassing two overlapping binding sites

Released:
DOI: 10.2210/pdb8c7s/pdb
PDB entry 8c7s coloured by chain, front view.
PDB entry 8c7s coloured by chain, side view.
PDB entry 8c7s coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into CodY activation and DNA recognition.
Hainzl T, Bonde M, Almqvist F, Johansson J, Sauer-Eriksson AE
OpenAccess logo Nucleic Acids Res (2023)
PMID: 37326020

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235634 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Global transcriptional regulator CodY Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 28.67 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FHI3 (Residues: 1-256; Coverage: 100%)
Gene names: SAUSA300_1148, codY
Sequence domains:
DNA (30-MER) Chain: M
Molecule details ›
Chain: M
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
DNA (30-MER) Chain: N
Molecule details ›
Chain: N
Length: 30 nucleotides
Theoretical weight: 9.26 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand ILE 2 x ILE
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6122
Unit cell:
a: 104.112Å b: 104.112Å c: 257.226Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links