› 8c8t

Electron Microscopy
3.2Å resolution

cryo-EM structure of BG505 SOSIP.664 HIV-1 Env trimer in complex with bNAbs EPTC112 and 3BNC117

Released:
DOI: 10.2210/pdb8c8t/pdb
PDB entry 8c8t coloured by chain, front view.
PDB entry 8c8t coloured by chain, side view.
PDB entry 8c8t coloured by chain, top view.
Source organisms:
Primary publication:
Anti-V1/V3-glycan broadly HIV-1 neutralizing antibodies in a post-treatment controller.
Molinos-Albert LM, Baquero E, Bouvin-Pley M, Lorin V, Charre C, Planchais C, Dimitrov JD, Monceaux V, Vos M, Hocqueloux L, Berger JL, Seaman MS, Braibant M, Avettand-Fenoël V, Sáez-Cirión A, Mouquet H
Cell Host Microbe (2023)
PMID: 37433296
Related structures: EMD-16493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274313 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (9 distinct):
Envelope glycoprotein gp160 Chains: A, F, G
Molecule details ›
Chains: A, F, G
Length: 469 amino acids
Theoretical weight: 52.55 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 33-501; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Gp41 Bg505 T332n Sosip.664 Chains: B, I, J
Molecule details ›
Chains: B, I, J
Length: 145 amino acids
Theoretical weight: 16.43 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
IgG EPTC112 Fab fragment heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 233 amino acids
Theoretical weight: 25.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
IgG 3BNC117 Fab heavy chain Chains: R, S
Molecule details ›
Chains: R, S
Length: 226 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
IgG 3BNC117 Fab Light Chain Chains: T, U
Molecule details ›
Chains: T, U
Length: 206 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
IgG EPTC112 Fab Light Chain Chains: D, K
Molecule details ›
Chains: D, K
Length: 216 amino acids
Theoretical weight: 22.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 24 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-16493
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Early antiretroviral therapy favors post-treatment SIV control associated with the expansion of enhanced memory CD8+ T-cells.
Passaes et al. (2024)