› 8c8w

X-ray diffraction
2.29Å resolution

Priestia megaterium mupirocin hyper-resistant HIGH motif mutant of type 2 isoleucyl-tRNA synthetase complexed with an isoleucyl-adenylate analogue

Released:
DOI: 10.2210/pdb8c8w/pdb
PDB entry 8c8w coloured by chain, front view.
PDB entry 8c8w coloured by chain, side view.
PDB entry 8c8w coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Antibiotic hyper-resistance in a class I aminoacyl-tRNA synthetase with altered active site signature motif.
Brkic A, Leibundgut M, Jablonska J, Zanki V, Car Z, Petrovic Perokovic V, Marsavelski A, Ban N, Gruic-Sovulj I
OpenAccess logo Nat Commun 14 5498 (2023)
PMID: 37679387

Function and Biology Details

Reaction catalysed: 
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-493088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoleucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 1032 amino acids
Theoretical weight: 117.86 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B6AVD3 (Residues: 1-1032; Coverage: 100%)
Gene names: BG04_5198, ileS
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ILA 1 x ILA
Ligand TAR 4 x TAR
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P43212
Unit cell:
a: 107.92Å b: 107.92Å c: 239.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.231
Expression system: Escherichia coli BL21(DE3)

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