› 8c9s

X-ray diffraction
1.8Å resolution

Catechol O-methyltransferase from Streptomyces avermitilis in complex with SAH

Released:
DOI: 10.2210/pdb8c9s/pdb
PDB entry 8c9s coloured by chain, front view.
PDB entry 8c9s coloured by chain, side view.
PDB entry 8c9s coloured by chain, top view.
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Primary publication:
Structural characterization and extended substrate scope analysis of two Mg2+-dependent O-methyltransferases from bacteria.
Sokolova N, Zhang L, Deravi S, Oerlemans R, Groves MR, Haslinger K
Chembiochem e202300076 (2023)
PMID: 36942619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.22 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82B68 (Residues: 1-224; Coverage: 100%)
Gene name: SAVERM_5837
Sequence domains: O-methyltransferase

Ligands and Environments

3 bound ligands:
Ligand SAH 2 x SAH
Ligand MG 2 x MG
Ligand BAQ 2 x BAQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 46.398Å b: 41.596Å c: 104.207Å
α: 90° β: 96.564° γ: 90°
R-values:
R R work R free
0.164 0.162 0.211
Expression system: Escherichia coli BL21(DE3)

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