8cec

Electron Microscopy
3.57Å resolution

Rnase R bound to a 30S degradation intermediate (State I - head-turning)

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-249722 (preferred)
Entry contents:
20 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (22 distinct):
Ribonuclease R Chain: C
Molecule details ›
Chain: C
Length: 779 amino acids
Theoretical weight: 88.89 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: O32231 (Residues: 1-779; Coverage: 100%)
Gene names: BSU33610, rnr, vacB, yvaJ
Sequence domains:
Small ribosomal subunit protein uS2 Chain: D
Molecule details ›
Chain: D
Length: 246 amino acids
Theoretical weight: 28.01 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P21464 (Residues: 1-246; Coverage: 100%)
Gene names: BSU16490, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS4 Chain: F
Small ribosomal subunit protein uS5 Chain: G
Small ribosomal subunit protein uS8 Chain: I
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS20 Chain: S
Small ribosomal subunit protein bS6 Chain: T
Small ribosomal subunit protein bS18 Chain: U
Small ribosomal subunit protein uS11 Chain: V
Small ribosomal subunit protein uS3 Chain: H
Small ribosomal subunit protein uS7 Chain: K
Small ribosomal subunit protein uS9 Chain: N
Small ribosomal subunit protein uS10 Chain: R
Small ribosomal subunit protein uS13 Chain: X
Small ribosomal subunit protein uS14B Chain: Y
Small ribosomal subunit protein uS19 Chain: a
16S ribosomal RNA Chain: A
RNA Substrate Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.57Å
Relevant EMDB volumes: EMD-16605