› 8ci2

Electron Microscopy
4.4Å resolution

human alpha7 nicotinic receptor in complex with the C4 nanobody under sub-saturating conditions

Released:
DOI: 10.2210/pdb8ci2/pdb
PDB entry 8ci2 coloured by chain, front view.
PDB entry 8ci2 coloured by chain, side view.
PDB entry 8ci2 coloured by chain, top view.
Source organisms:
Primary publication:
An original potentiating mechanism revealed by the cryo-EM structures of the human α7 nicotinic receptor in complex with nanobodies.
Prevost MS, Barilone N, Dejean de la Bâtie G, Pons S, Ayme G, England P, Gielen M, Bontems F, Pehau-Arnaudet G, Maskos U, Lafaye P, Corringer PJ
OpenAccess logo Nat Commun 14 5964 (2023)
PMID: 37749098
Related structures: EMD-16666

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231801 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuronal acetylcholine receptor subunit alpha-7 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 388 amino acids
Theoretical weight: 44.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P36544 (Residues: 24-347, 455-502; Coverage: 78%)
  • Best match: P36544-3 (Residues: 24-83, 43-79)
Gene names: CHRNA7, NACHRA7
Sequence domains:
Nanobody C4 Chains: F, G, I
Molecule details ›
Chains: F, G, I
Length: 147 amino acids
Theoretical weight: 15.83 KDa
Source organism: Vicugna pacos
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 10 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-16666
Expression system: Homo sapiens

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