› 8cix

X-ray diffraction
1.76Å resolution

DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Griselimycin.

Released:
DOI: 10.2210/pdb8cix/pdb
PDB entry 8cix coloured by chain, front view.
PDB entry 8cix coloured by chain, side view.
PDB entry 8cix coloured by chain, top view.
Source organisms:
Primary publication:
Elucidation of unusual biosynthesis and DnaN-targeting mode of action of potent anti-tuberculosis antibiotics Mycoplanecins.
Fu C, Liu Y, Walt C, Rasheed S, Bader CD, Lukat P, Neuber M, Haeckl FPJ, Blankenfeldt W, Kalinina OV, Müller R
OpenAccess logo Nat Commun 15 791 (2024)
PMID: 38278788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236067 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 40.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Griselimycin Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand GOL 1 x GOL
Ligand CA 7 x CA
Ligand ACT 3 x ACT
Ligand MG 4 x MG
Ligand NA 5 x NA
3 modified residues:
Ligand MVA 2 x MVA
Ligand MP8 2 x MP8
Ligand NZC 1 x NZC

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C222
Unit cell:
a: 83.936Å b: 150.561Å c: 71.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.205
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links