› 8cj9

X-ray diffraction
1.93Å resolution

Crystal structure of maize CKO/CKX8 in complex with urea-derived inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]benzamide

Released:
DOI: 10.2210/pdb8cj9/pdb
PDB entry 8cj9 coloured by chain, front view.
PDB entry 8cj9 coloured by chain, side view.
PDB entry 8cj9 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice.
Nisler J, Klimeš P, Končitíková R, Kadlecová A, Voller J, Chalaki M, Karampelias M, Murvanidze N, Werbrouck SPO, Kopečný D, Havlíček L, de Diego N, Briozzo P, Moréra S, Zalabák D, Spíchal L
J Exp Bot (2024)
PMID: 38776394

Function and Biology Details

Reaction catalysed: 
N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cytokinin dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 539 amino acids
Theoretical weight: 58.87 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: E3T1X2 (Residues: 1-539; Coverage: 100%)
Gene names: 100526747, CKX8
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand UZX 2 x UZX
Ligand GOL 2 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 45.78Å b: 50.69Å c: 110.44Å
α: 92.43° β: 90.09° γ: 108.77°
R-values:
R R work R free
0.19 0.188 0.224
Expression system: Escherichia coli

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