› 8cny

X-ray diffraction
1.51Å resolution

Structure of Enterovirus A71 3C protease

Released:
DOI: 10.2210/pdb8cny/pdb
PDB entry 8cny coloured by chain, front view.
PDB entry 8cny coloured by chain, side view.
PDB entry 8cny coloured by chain, top view.
Source organism: Enterovirus
Entry authors: Lithgo RM, Fairhead M, Koekemoer L, Aschenbrenner JC, Balcomb BH, Godoy AS, Marples PG, Ni X, Tomlinson CWE, Wild C, Fearon D, Walsh MA, von Delft F

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C protease Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.36 KDa
Source organism: Enterovirus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B8YLV8 (Residues: 1-183; Coverage: 100%)
Sequence domains: 3C cysteine protease (picornain 3C)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 64.9Å b: 65.73Å c: 76.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.21
Expression system: Escherichia coli BL21

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