› 8cpd

Electron Microscopy
3.46Å resolution

Cryo-EM structure of CRaf dimer with 14:3:3

Released:
DOI: 10.2210/pdb8cpd/pdb
PDB entry 8cpd coloured by chain, front view.
PDB entry 8cpd coloured by chain, side view.
PDB entry 8cpd coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of CRAF2/14-3-32 and CRAF2/14-3-32/MEK12 complexes.
Dedden D, Nitsche J, Schneider EV, Thomsen M, Schwarz D, Leuthner B, Grädler U
J Mol Biol 168483 (2024)
PMID: 38331211
Related structures: EMD-16779

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269715 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RAF proto-oncogene serine/threonine-protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 648 amino acids
Theoretical weight: 73.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04049 (Residues: 1-648; Coverage: 100%)
Gene names: RAF, RAF1
Sequence domains:
14-3-3 domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 247 amino acids
Theoretical weight: 28.11 KDa
Source organism: Spodoptera frugiperda
UniProt:
  • Canonical: A0A9R0D7T1 (Residues: 1-247; Coverage: 100%)
Gene name: LOC118271767
Sequence domains: 14-3-3 protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-16779
Expression system: Spodoptera frugiperda

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