› 8cr4

X-ray diffraction
0.91Å resolution

Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816

Released:
DOI: 10.2210/pdb8cr4/pdb
PDB entry 8cr4 coloured by chain, front view.
PDB entry 8cr4 coloured by chain, side view.
PDB entry 8cr4 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Facile Production of the Pseudomonas aeruginosa Virulence Factor LasB in E. coli for Structure-Based Drug Design.
Kolling D, Haupenthal J, Hirsch AKH, Köhnke J
Chembiochem e202300185 (2023)
PMID: 37195753

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins including elastin, collagen types III and IV, fibronectin and immunoglobulin A, generally with bulky hydrophobic group at P1'. Insulin B chain cleavage pattern identical to that of thermolysin, but specificity differs in other respects.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elastase Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 55.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14756 (Residues: 24-498; Coverage: 100%)
Gene names: PA3724, lasB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 40.405Å b: 40.464Å c: 45.376Å
α: 100.515° β: 98.608° γ: 107.449°
R-values:
R R work R free
0.195 0.194 0.206
Expression system: Escherichia coli

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