› 8cso

X-ray diffraction
2.6Å resolution

Crystal Structure of Orotidine 5'-phosphate decarboxylase from Klebsiella pneumoniae in complex with Uridine-5'-monophosphate

Released:
DOI: 10.2210/pdb8cso/pdb
PDB entry 8cso coloured by chain, front view.
PDB entry 8cso coloured by chain, side view.
PDB entry 8cso coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae HS11286
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 236 amino acids
Theoretical weight: 25.69 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae HS11286
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3GNE4 (Residues: 2-228; Coverage: 100%)
Gene names: KPHS_21850, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family

Ligands and Environments

2 bound ligands:
Ligand U5P 5 x U5P
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 61.71Å b: 91.31Å c: 127.73Å
α: 100.942° β: 100.364° γ: 91.113°
R-values:
R R work R free
0.198 0.197 0.235
Expression system: Escherichia coli BL21(DE3)

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